0.25ml 0.5ml 1ml Ƙaramin Ƙananan Jini na Tarin Jini

Bututun tattara jini mai ƙananan girma yana da tsari mai kyau da kuma murfin kariya mai rufewa, bututun zai iya hana zubar jini yadda ya kamata. Saboda tsarinsa mai haƙori da yawa da kuma tsarin daidaitawa biyu, yana da sauƙin jigilar shi cikin aminci da kuma sauƙin aiki, ba tare da zubar jini ba.

Lambar launi ta murfin aminci ta yi daidai da Ma'aunin Ƙasa da Ƙasa, Mai Sauƙin ganewa.

Tsarin bakin bututun mai kyau yana da sauƙi ga masu amfani su zuba jini a cikin bututun. Mai sauƙi, sauri da fahimta, ana iya karanta adadin jinin cikin sauƙi tare da layin kammala karatu mai haske.

An yi masa magani na musamman a cikin bututun, yana da santsi a saman ba tare da mannewa da jini ba.

Za a iya keɓance lambar barcode, kuma a tsaftace bututun da haskoki na gamma kamar yadda abokan ciniki ke buƙata don cimma gwajin asepsis.

1. Bututun Mai kunna Gel & Clot

Ana amfani da bututun gel da bututun activator don biochemistry na jini, ilimin rigakafi da gwajin magunguna, da sauransu. A can ana fesa coagulant ɗin a saman bututun, wanda zai rage lokacin clotting sosai.

Ganin cewa gel ɗin rabuwa da aka shigo da shi daga Japan tsantsar abu ne, mai karko a fannin kimiyyar sinadarai, yana iya jure zafin jiki mai yawa don haka gel ɗin zai ci gaba da kasancewa cikin kwanciyar hankali yayin ajiya da jigilar kaya.

Gel ɗin zai yi ƙarfi bayan an yi amfani da shi wajen centrifugation sannan ya raba sinadarin jini gaba ɗaya daga ƙwayoyin fibrin kamar wani shinge, wanda hakan ke hana musayar sinadarai tsakanin sinadarin jini da ƙwayoyin halitta yadda ya kamata. Ana inganta ingancin tarin sinadarin jini kuma za a sami ingantaccen sakamako na gwaji, don haka ana samun sakamako mafi inganci.

A bar sinadarin ya tsaya na tsawon fiye da awanni 48, babu wani canji da zai faru a zahiri game da siffofin sinadarai da abubuwan da ke cikin sinadarai, sannan a yi amfani da bututun kai tsaye a cikin na'urorin tantance samfurin.

- Lokacin da za a cire gaba ɗaya daga jini: minti 20-25
- Saurin centrifugation: 3500-4000r/m
- Lokacin centrifugation: minti 5
- Zafin ajiya da aka ba da shawarar: 4-25ºC

2. Tube Mai kunna clot

Ana amfani da bututun kunna jini a cikin tarin jini don nazarin halittu da kuma ilimin rigakafi a cikin duba lafiya. Ya dace da yanayin zafin aiki mai yawa. Tare da magani na musamman, saman ciki na bututun yana da santsi sosai inda ake fesa sinadarin coagulant mai inganci iri ɗaya. Samfurin jinin zai taɓa coagulant gaba ɗaya da kuma clotting cikin mintuna 5-8. Don haka ana samun sinadarin serum mai inganci ta hanyar centrifugation daga baya, ba tare da fashewa daga ƙwayoyin jini ba, hemolysis, rabuwa da furotin fibrin, da sauransu.

Saboda haka, maganin zai iya biyan buƙatun asibiti mai sauri da gwajin gaggawa na maganin.
- Lokacin da za a cire gaba ɗaya daga jini: minti 20-25
- Saurin centrifugation: 3500-4000r/m
- Lokacin centrifugation: minti 5
- Zafin ajiya da aka ba da shawarar: 4-25ºC

3. Jirgin Ruwa na EDTA

Ana amfani da bututun EDTA sosai a fannin ilimin jinin jini, daidaita jini, rarraba jini da kuma nau'ikan kayan aikin gwajin ƙwayoyin jini daban-daban.

Yana bayar da cikakken kariya ga ƙwayoyin jini, musamman don kare ƙwayoyin jini, ta yadda zai iya dakatar da taruwar ƙwayoyin jini yadda ya kamata kuma ya sa siffar da girman ƙwayoyin jini ba su da tasiri cikin dogon lokaci.

Kayan ado masu kyau tare da dabarar super-minute za su iya fesa ƙarin a saman bututun gaba ɗaya, don haka samfurin jini zai iya haɗuwa gaba ɗaya da ƙarin. Ana amfani da plasma na hana ɗaukar jini na EDTA don gwajin halittu na ƙwayoyin cuta masu cutarwa, ƙwayoyin cuta da ƙwayoyin cuta, da sauransu.

4.Tushen DNA

1. An cika bututun RNA/DNA na jini da wani reagent na musamman don kare RNA/DNA na samfuran da sauri don kada su lalace.

2. Ana iya adana samfuran jinin na tsawon kwana 3 a zafin 18-25°C, a adana na tsawon kwanaki 5 a zafin 2-8°C, a ajiye su na tsawon akalla watanni 50 a -20°C zuwa -70°C.

3. Yana da sauƙin amfani, kawai a juya bututun RNA/DNA na jini sau 8 bayan an tattara shi sosai.

4. A shafa a jinin mutane da dabbobi masu shayarwa, wanda bai dace da jinin da ya tsufa ba da kuma jinin da ke narkewa da kuma jinin kaji da sauran dabbobi.

5. Tattara, adanawa da jigilar samfuran gano RNA/DNA na jini gaba ɗaya daidai gwargwado

6. Bangon ciki na bututun yana da tsari na musamman ba tare da RNase, DNase ba, yana tabbatar da cewa samfuran gano ƙwayoyin nucleic acid suna da inganci.

7. Yana taimakawa wajen fitar da samfura cikin sauri da kuma yawan aiki, yana inganta aikin dakin gwaje-gwaje

5.Tube na ESR

Ana amfani da bututun ESR na Ø13×75mm musamman wajen tattara jini da kuma hana zubar jini don gwajin ƙimar sedimentation na Erythrocyte mai sarrafa kansa tare da rabon haɗuwa na kashi 1 na sodium citrate zuwa sassa 4 na jini, ta hanyar hanyar Westergren.

6. Bututun Glucose

Ana amfani da bututun glucose wajen tattara jini don gwajin kamar sukari a jini, jure wa sukari, electrophoresis na erythrocyte, haemoglobin na hana alkali da lactate. Ƙarin Sodium Fluoride yana hana metabolism na sukari a jini yadda ya kamata kuma Sodium Heparin yana magance hemolysis cikin nasara.

Saboda haka, asalin yanayin jini zai daɗe na dogon lokaci kuma ya tabbatar da ingantaccen gwajin sukari a cikin awanni 72. Ƙarin da za a iya ƙarawa shine Sodium Fluoride+Sodium Heparin, Sodium Fluoride+ EDTA.K2, Sodium Fluoride+EDTA.Na2.

Gudun centrifugation: 3500-4000 r/m
Lokacin centrifugation: minti 5
Zafin ajiya da aka ba da shawarar: 4-25 ºC

7. Jirgin Heparin
Ana amfani da bututun Heparin wajen tattara jini don gwajin jini na asibiti, biochemistry na gaggawa da rheology na jini, da sauransu. Ba tare da wani tsangwama ga abubuwan da ke cikin jini ba kuma ba shi da wani tasiri ga girman erythrocyte, ba zai haifar da hemolysis ba. Bugu da ƙari, yana da fasalulluka na rabuwar plasma cikin sauri da kewayon zafin aiki mai yawa da kuma babban jituwa da ma'aunin jini.

Heparin na hana zubar jini yana kunna fibrinolysin, yayin da yake hana thromboplastin, sannan ya cimma daidaito tsakanin fibrinogen da fibrin, ba tare da zaren fibrin ba a cikin tsarin dubawa. Yawancin ma'aunin plasma za a iya maimaita su cikin awanni 6.

Lithium heparin ba wai kawai yana da siffofin sodium heparin ba, har ma ana iya amfani da shi a gwajin ƙananan abubuwa ba tare da wani tasiri ga sodium ion ba. Don biyan buƙatun dakin gwaje-gwaje daban-daban, KANGJIAN na iya ƙara gel ɗin rabuwar plasma don yin plasma mai inganci.

Gudun centrifugation: 3500-4000 r/m
Lokacin centrifugation: minti 3
Zafin ajiya da aka ba da shawarar: 4-25ºC

8. PT Tube

Ana amfani da bututun PT don gwajin coagulation na jini kuma yana aiki akan tsarin fibrinolytic (PT, TT, APTT da fibrinogen, da sauransu).
Rabon hadawa shine kashi 1 na citrate zuwa kashi 9 na jini. Daidaitaccen rabon zai iya tabbatar da ingancin sakamakon gwajin kuma ya hana a gano cutar da ba ta dace ba.

Ganin cewa sodium citrate ba shi da guba sosai, ana amfani da shi don adana jini. A yi amfani da isasshen jini don tabbatar da ingantaccen sakamakon gwaji. Bututun PT mai bene biyu yana da ɗan ƙaramin sarari, wanda za a iya amfani da shi don sa ido kan gwajin v WF, F, ayyukan platelet, da maganin Heparin.