Menene EDTA Tarin Jini kuma Yaya Ake Amfani da Su?

A cikin gwajin likita da ganewar asibiti da magani,EDTA tarin jini, a matsayin mahimman abubuwan da ake amfani da su don tarin jini, suna taka muhimmiyar rawa wajen tabbatar da amincin samfuran da daidaiton gwaji. A cikin wannan labarin, za mu yi nazari sosai kan wannan "masanin ganuwa" a fannin likitanci daga sassan ma'anar, rabe-raben launi, ka'idar anticoagulation, manufar gwaji da amfani da daidaitattun.

MeneneEDTA tarin jini?

EDTA bututun tarin jini wani nau'i ne na bututun tarin jini mai dauke da Ethylene Diamine Tetraacetic Acid ko gishirin sa, wanda ake amfani da shi musamman don tarin samfuran jini da maganin hana jini. EDTA na iya toshe maganin coagulation cascade ta hanyar chelating ions na calcium a cikin jini, don kiyaye jinin a cikin ruwa na dogon lokaci, da samar da samfurori masu tsayayye don gwaje-gwaje na yau da kullun na jini da ilimin halitta. Yana ba da tabbataccen samfura don aikin yau da kullun na jini, ilimin halitta da sauran gwaje-gwaje.

A matsayin muhimmin sashi namagunguna masu amfani, EDTA tubes tarin jini yana buƙatar bin ka'idodin ƙasa na "Amfani guda ɗaya na venous jini tarin kwantena" (misali GB / T 19489-2008) don tabbatar da aikin haihuwa, marasa pyrogenic da rashin cytotoxicity.

Launuka daban-daban na EDTA tarin jini

Dangane da ƙa'idodin gama gari na duniya (irin su jagororin CLSI H3-A6), EDTA tarin tarin jini yawanci ana rufe su da shuɗi (EDTA-K2/K3) ko shuɗi (sodium citrate gauraye da EDTA) don bambanta amfani:

Lura: Wasu samfuran ƙila za a iya ƙididdige su a wasu launuka, duba umarnin kafin amfani.

Tsarin hana zubar jini na EDTA tubes tarin jini

EDTA ta hanyar rukunin carboxyl na kwayoyin halitta (-COOH) da ions calcium a cikin jini (Ca²⁺) sun haɗu don samar da tsayayyen chelate, don haka hana kunna plasminogen, toshe tsarin coagulation na fibrinogen cikin fibrin. Wannan anticoagulation yana da halaye masu zuwa:

1. saurin farawa na aiki: ana iya kammala maganin rigakafi a cikin minti 1-2 bayan tarin jini;

2. babban kwanciyar hankali: ana iya adana samfurori fiye da sa'o'i 48 (ana iya ƙarawa a cikin firiji zuwa sa'o'i 72);

3. Faɗin aikace-aikacen: dace da yawancin gwaje-gwajen jini, amma ba don gwajin aikin coagulation ko platelet (ana buƙatar bututun sodium citrate).

Abubuwan gwaji masu mahimmanci na bututun tarin jini na EDTA

1. Binciken jini na yau da kullun: adadin fararen jinin jini, sigogin jinin jajayen jini, tattarawar haemoglobin, da sauransu;

2. Ƙididdigar ƙungiyar jini da haɗin kai: Ƙungiyar jini na ABO, Rh factor gano;

3. ganewar asali na kwayoyin: gwajin kwayoyin halitta, ƙaddarar nauyin ƙwayar cuta (misali HIV, HBV);

4. glycated haemoglobin (HbA1c): dogon lokaci na lura da glucose na jini don ciwon sukari mellitus;

5. gwajin jini na jini: Plasmodium, gano microfilariae.

Amfani da ka'idoji da kariya

1. Tsarin tattarawa:

Bayan kawar da fata, yi aiki bisa ga ma'auni na tarin jini na venous;

Nan da nan bayan tattarawa, jujjuya bututun tarin jini sau 5-8 don tabbatar da cewa maganin rigakafin ya haɗu da jini sosai;

Guji girgizawa mai ƙarfi (don hana hemolysis).

2. Adana da sufuri:

Ajiye a dakin da zafin jiki (15-25 ° C), kauce wa zafi ko daskarewa;

Sanya a tsaye yayin sufuri don hana sassauta hular bututu.

3. contraindications scenarios:

Ana buƙatar bututun citrate na sodium don Coagulation IV (PT, APTT, da dai sauransu);

Gwajin aikin platelet yana buƙatar bututun citrate sodium.

Yadda za a zabi high qualityEDTA tarin jini?

1. Cancanta da takaddun shaida: zaɓi samfuran da suka wuce ISO13485 da takaddun CE. 2;

2. Tsaro na kayan abu: jikin bututu ya kamata ya kasance mai haske kuma ba tare da ragowar filastik ba;

3. Matsakaicin daidaituwa: adadin maganin da aka ƙara ya kamata ya kasance daidai da daidaitattun ƙasa (misali EDTA-K2 maida hankali na 1.8 ± 0.15mg / mL);

4. Sunan alama: Ana ba da fifiko ga sanannun samfuran masana'antu a fagen kayan aikin likitanci don tabbatar da kwanciyar hankali.

Kammalawa

A matsayin maɓalli nana'urar tattara jini, EDTA tubes na tarin jini suna da tasiri kai tsaye akan daidaiton sakamakon gwajin dangane da abubuwan da suka dace da su. Ta hanyar daidaita amfani da bututun tarin jini masu launi daban-daban da kuma haɗa su tare da tsauraran hanyoyin tattarawa, zai iya samar da ingantaccen tushe don ganewar asibiti. A nan gaba, tare da ci gaba da ingantaccen magani, EDTA tarin jini zai taka muhimmiyar rawa wajen nazarin jini, jerin kwayoyin halitta da sauran fannoni, da kuma ci gaba da kare lafiyar ɗan adam.